Catheter-Associated Urinary Tract Infection (CAUTI) is 
common worldwide health care associated problem. 
Antibiotic resistance in CAUTI is due to wide spread use of 
urinary catheter and inappropriate use of antibiotics. The 
present study highlights the trend of organism and their 
resistance patterns. This observational prospective study was done on 100 
CAUTI patients in Comilla medical college hospital, 
Comilla, Bangladesh. Urine culture positive patients 
collected by suprapubic puncture meet the criteria of 
CAUTI with formation of biofilm in catheter was selected.  
E.coli was the most frequently isolated pathogen in both 
urine and biofilm (60% vs.72%) followed by Klebsiella and 
others. Biofilm strains displayed relatively high resistance 
against tested antibiotics. The E.coli in both urine and 
biofilm shows high resistance against ciprofloxacin and 
cotrimoxazole (80%& 65% vs 84% & 82% respectively), 
and low for imipenem and amikacin (5% and 18% vs 8% 
and19% respectively). Urine and catheter biofilm resistant 
to all tested drugs was observed for E.coli in 6.95% and 
3.33% cases respectively.  
E.coli was the most frequent isolate followed Klebsiella and 
others. E. coli in both urine and biofilm showed the high 
resistance to the quinolones and cotrimoxazole and low for carbapenems and amikacin.