Objective to study the effect of doxorubicin 
interventional chemotherapy on rabbit VX2 renal 
transplantation carcinoma and its mechanism. Methods 
Thirty healthy New Zealand white rabbits were chosen to 
establish VX2 renal transplantation carcinoma models. 
The experimental rabbits were randomly divided into 
three groups with 10 rabbits in each group. The rabbits in 
the control group (negative control), doxorubicin group 
and cisplatin group were treated with saline, 5mg/kg 
doxorubicin and 2mg/kg cisplatin respectively. The 
tumor volume was monitored with B-mode 
ultrasonography. The rabbits were anesthetized and killed 
after two weeks of interventional chemotherapy. The 
changes of Bcl-2 and Bax at the levels of mRNA and 
protein were analyzed with real-time PCR and 
immunehistochemistry. Results The efficacy of 
interventional chemotherapy was evaluated with tumor 
volume changes monitored by B-mode ultrasonography. 
The tumor volume of control group and doxorubicin 
group 
was 1.29±0.60cm3 and 0.47±0.12 cm3 
respectively. Further fluorescence quantitative PCR 
detection results showed that doxorubicin could reduce 
the Bcl-2 expression and increase the Bax expression 
(P<0.05).The result of immunohistochemistry was 
consistent with that of fluorescence quantitative PCR. 
Conclusions The effect of doxorubicin interventional chemotherapy on renal transplantation carcinoma is 
obvious and the mechanism may be related to the down
regulation of Bcl-2 expression and up-regulation of Bax 
expression thus inducing the apoptosis of tumor cells