We previously reported that the largest p150 subunit of chromatin assembly factor 1 (p150CAF1), which promotes chromatin assembly in the context of DNA replication, interacts directly and preferentially with small ubiquitin-related modifier-2/3 (SUMO-2/3). In this study, using 5-ethynyl-2′-deoxyuridine, we demonstrate accumulation of SUMO-2/3 and the ubiquitin ligase RNF4 at DNA replication sites in a human cell line stably over expressing Flag-tagged p150CAF1 (Flag-p150CAF1 cells). As the SUMO-RNF4 pathway remains elusive, this cell line may serve as a valuable tool for clarifying the pathway regulation and function in chromatin dynamics
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ISSN: 2470-1114
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Original Research
Vol. 1, Issue 1 (2015)
Sumo-2/3 and Rnf4 Localization to DNA Replication Foci in Cultured Human Cells Over expressing the P150 Subunit of Chromatin Assembly Factor 1
Eri Yuasa and Hisato Saitoh*
Corresponding Author: Eri Yuasa and Hisato Saitoh*
Received: Sep 13, 2014
Accepted: Oct 20, 2014
Published: Oct 24, 2014
Views: 12
DOI: 10.14437
Abstract
Eri Yuasa and Hisato Saitoh (2014), Sumo-2/3 and Rnf4 Localization to DNA Replication Foci in Cultured Human Cells Over expressing the P150 Subunit of Chromatin Assembly Factor 1. Aperito J Cell Mol Biol 1:101
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