Enhanced susceptibility of amyl nitrite or butyl nitrite 
induced oxidation of oxyhemoglobin into methemoglobin in the 
Type 2 diabetics blood compared to normal blood in independent 
studies was confirmed (P<0.05). For amyl nitrite induced 
oxidation of hemoglobin a linear regression line was calculated 
from 40 donors, 20 of whom were diabetics and 20 of whom were 
normal or non diabetics, using all their HbA1C (%) values and the 
measured hemoglobin oxidation times. The resultant equation was 
that of a straight line which is most accurately described by the 
equation Y = 4.43 - 0.25X where X = percent of HbA1C and Y = 
hemoglobin oxidation time in minutes. The standard deviation of 
points around the fitted line was 0.449 and the standard errors of 
the slope and intercept were 0.0230 and 0.207, respectively. The 
product moment correlation coefficient (r) was found to be - 0.87. 
For butyl nitrite induced oxidation of hemoglobin another linear 
regression line was calculated from all donor HbA1C values and 
all of the measured hemoglobin oxidation times from another 40 
donors, 20 of whom were diabetics and 20 of whom were normal. 
The resultant equation was that of a straight line which is most 
accurately described by the equation Y = 7.17 - 0.53X where X = 
percent of HbA1C and Y = hemoglobin oxidation time in 
minutes. The standard deviation of points around the fitted line 
was 1.00 and the standard errors of the slope and intercept were 
0.0586 and 0.472, respectively. The product moment correlation 
coefficient (r) was found to be - 0.83. Thus, based on the fact that 
both correlation coefficients are less than -0.75 this study 
demonstrates that there is a very good to excellent inverse 
relationship between HbA1C percentage and the hemoglobin 
oxidation time for both amyl nitrite and butyl nitrite. This similar 
finding could be attributed to the fact that these alkyl nitrites